INSTITUT DE BIOLOGIE DU DEVELOPPEMENT DE MARSEILLE

Actualités

A novel method to image gene expression in vivo

Antoine Barrière and Amel Toudji-Zouaz in the Bertrand team developed a new method to image and quantify transcription in vivo.
Date de publication

Antoine Barrière and Amel Toudji-Zouaz in the Bertrand team developed a new method to image and quantify transcription in vivo.

To understand how cells acquire their identity during development, it is essential to follow gene expression dynamics in vivo. Antoine Barrière and Amel Toudji-Zouaz in the Bertrand team developed a new method to image transcription using the Argonaute protein NRDE-3 tagged with a fluorescent protein. NRDE-3 is programmed with dsRNA targeting a gene of interest and labels nascent transcripts at transcription sites and mRNA in the cytoplasm. In C. elegans, dsRNA are easily introduced by feeding the animals. Expression of the gene of interest is then followed and quantified over time by in vivo fluorescent microscopy.

Toudji-Zouaz-et-al
(A) Principle of the method.
(B) Labelling of transcription sites (red arrows) of hlh-1/MyoD in muscle cells of a C. elegans embryo

To know more :

Contact

Antoine Barrière: antoine.barriere@univ-amu.fr
Vincent Bertrand: vincent.bertrand@univ-amu.fr

Catégories

Dernières publications

Show me your rhythm!

We introduce an algorithm, Phasik, for extracting the phases of biological systems by clustering partial temporal networks.

AGENDA

OFFRES D'EMPLOI

TWITTER

02/02/2023

How disruption drives change

Interaction between tight junction formation and gastrulation signals regulates mesendoderm differentiation in hiPSCs

01/02/2023

Show me your rhythm!

We introduce an algorithm, Phasik, for extracting the phases of biological systems by clustering partial temporal networks.